Duggar, et al. had originally described the characters of S. aureofaciens in their patent as seen also in Waksman’s book²⁾ and they reported its variation range on various media. ^{3, 4} Boretti, et al⁵⁾. and Scotti, et al⁶⁾. had reported cultural or cytological studies of S. aureofaciens with regards of chlortetracycline production.

The author isolated producers of chlortetracycline from soil and examined their cultural characters and cytological features of those organisms. In this paper, he presents the identity of his organisms with S. aureofaciens and some cultural and cytological findings which could be concerned with antibiotic production.