The isolation, characterization, and biological activities from carzinostatin were already described in a preceding paper¹⁾. When tested against Ehrlich ascites carcinoma, both culture filtrates and carzinostatin complex, the crude preparation obtained from the filtrates by adsorption and elution on acid clay, were found to exhibit marked antitumor effect. Purification of carzinostatin complex established against sarcoma 180, Bashford carcinoma 63, and ascitic form of mouse hepatoma 134, in addition to Ehrlich ascites carcinoma.